7. BMP多肽修饰HA-PLLA-明胶三维纳米纤维支架用于骨再生

7. BMP多肽修饰HA-PLLA-明胶三维纳米纤维支架用于骨再生

      制备出了一种同时具有纳米纤维结构和相互连通的多孔结构的支架材料，并应用于骨组织工程领域的研究。通过预先制备的静电纺纳米纤维膜材料，并结合匀浆技术，冷冻干燥法和热交联技术，成功地获得了纳米羟基磷灰石/PLLA/gelatin (nHA/PLA/GEL)有机无机复合的三维纳米纤维支架。随后，通过一种聚多巴胺涂层辅助方法，进一步将骨形态发生蛋白-2(BMP-2)的衍生肽负载到了支架上，并显示出了缓释的效果。支架在用于大鼠颅骨临界尺寸的缺损修复中表现出诱导骨再生的能力，植入体内2个月后基本修复了颅骨缺损。体内和体外的实验结果均表明：所制备的负载了BMP-2多肽的有机无机复合的纳米纤维三维多孔支架展现出了良好的生物相容性和骨诱导能力。

7. BMP polypeptide modified HA-PLLA-gelatin three-dimensional nanofiber scaffold for bone regeneration

A scaffold material having a nanofiber structure and interconnected porous structures was prepared and applied to the research in the field of bone tissue engineering. The nano-hydroxyapatite/PLLA/gelatin (nHA/PLA/GEL) organic-inorganic composite was successfully obtained by pre-prepared electrospun nanofiber membrane material combined with homogenization technology, freeze-drying method and thermal crosslinking technology. Subsequently, a derivative peptide of bone morphogenetic protein-2 (BMP-2) was further loaded onto the scaffold by a polydopamine coating-assisted method, and showed a sustained release effect. The scaffold showed the ability to induce bone regeneration in the defect repair of the critical size of the rat skull. After 2 months of implantation, the skull defect was basically repaired. The results of in vivo and in vitro experiments showed that the prepared organic-inorganic composite nanofiber three-dimensional porous scaffold loaded with BMP-2 polypeptide exhibited good biocompatibility and osteoinductivity.